Coding
RhaS
Part:BBa_K902068:Design
Designed by: Chris Wintersinger Group: iGEM12_Calgary (2012-10-02)
RhaS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 440
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 172
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part was inserted into pSB1C3 using Biobrick Assembly Standards.
Source
PCR amplified from Top Ten E. coli with Kapa polymerase.
References
Egan, S. M., Schleif, R. F., & others. (1993). A regulatory cascade in the induction of rhaBAD. Journal of Molecular Biology, 234(1), 87-98.